256, 6633–40 (1981)Įndo, T., Ohbayashi, H., Kanazawa, K., Kochibe, N., Kobata, A.: Carbohydrate binding specificity of immobilized Psathyrella velutina lectin. Kornfeld, S., Reitman, M.L., Kornfeld, R.: The carbohydrate-binding specificity of pea and lentil lectins. Merkle, R.K., Cummings, R.D.: Lectin affinity chromatography of glycoproteins. (Tokyo) 78, 687–96 (1975)īaenziger, J.U., Fiete, D.: Structural determinants of Ricinus communis agglutinin and toxin specificity for oligosaccharides. Ogata, S., Muramatsu, T., Kobata, A.: Fractionation of glycopeptides by affinity column chromatography on concanavalin A-Sepharose. 487, 7–15 (2004)Įpstein, C.J., Martin, G.M., Schultz, A.L., Motulsky, A.G.: Werner’s syndrome: A review of its symptomatology, natural history, pathologic features, genetics and relationship to the natural aging process. Ishibashi, Y., Kobayashi, F., Idesawa, A., Taniguchi, A., Matsuzawa, S.: Effects of carbocisteine on altered activities of glycosidases and glycosyltransferases and expression of Muc5ac in SO 2-exposed rats. Sato, T., Shirane, K., Kido, M., Furukawa, K.: Correlated gene expression between β-1,4-galactosyltransferase V and N-acetylglucosaminyltransferase V in human cancer cell lines. Yamamoto, K., Yamamoto, M., Ooka, H.: Changes in negative surface charge of human diploid fibroblasts, TIG-1, during in vitro aging. Sato, T., Furukawa, K., Greenwalt, D.E., Kobata, A.: Most bovine milk fat globule membrane glycoproteins contain asparagine-linked sugar chains with GalNAcβ1→4GlcNAc groups. 181, 257–67 (1997)įurukawa, K., Takamiya, K., Okada, M., Inoue, M., Fukumoto, S., Furukawa, K.: Novel functions of complex carbohydrates elucidated by the mutant mice of glycosyltransferase genes. Lu, Q., Hasty, P., Shur, B.D.: Targeted mutation in β1,4-galactosyltransferase leads to pituitary insufficiency and neonatal lethality. 13, 2056–65 (1994)Īsano, M., Furukawa, K., Kiso, M., Matsumoto, S., Umesaki, Y., Kochibe, N., Iwakura, Y.: Growth retardation and early death of β-1,4-galactosyltransferase-knockout mice with augmented proliferation and abnormal differentiation of epithelial cells. Metzler, M., Gertz, A., Sarkar, M., Schachter, H., Schrader, J.W., Marth, J.D.: Complex asparagine-linked oligosaccharides are required for morphogenic events during post-implantation development. Ioffe, E., Stanley, P.: Mice lacking N-acetylglucosaminyltransferase I activity die at mid-gestation, revealing an essential role for complex or hybrid N-linked carbohydrates. Kuwahara, I., Ikebuchi, K., Hamada, H., Niitsu, Y., Miyazawa, K., Ohyashiki, K., Fujisawa, H., Furukawa, K.: Changes in N-glycosylation of human stromal cells by telomerase expression. Katakura, Y., Nakata, E., Miura, T., Shirahata, S.: Transforming growth factor β triggers two independent-senescence programs in cancer cells. Kawado, T., Hayashi, O., Sato, T., Ito, H., Hayakawa, S., Takayama, E., Furukawa, K.: Rapid cell senescence-associated changes in galactosylation of N-linked oligosaccharides in human lung adenocarcinoma A549 cells. Greider, C.W.: Telomerase length regulation, Annu. 38, 1–48 (1987)īlackburn, E.H.: Structure and function of telomeres. Stanulis-Praeger, B.M.: Cellular senescence revisited: a review. Hayflick, L.: The limited in vitro lifetime of human diploid cell strains. Hayflick, L., Moorhead, P.S.: The serial cultivation of human diploid cell strains. These results indicate that the concordant decrease of the α-2,6-sialylation of N-glycans with the ST6Gal I gene expression is induced in TIG-3 cells by In support of this observation, the cDNA microarray assay and reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that the gene expression of the α-2,6-sialyltransferase I (ST6Gal I), which transfers sialic acid to galactose residues of N-glycans, decreases in the aged cells. Lectin blot analysis of membrane glycoproteins showed that the α-2-6-sialylation but not the α-2-3-sialylation of N-glycans decreases markedly in the aged cells when compared to the young cells. When the electrophoretic mobility of cells was determined, the negative surface charge of the aged cells decreased significantly when compared to that of young cells. Young cells (PDL 23) grew to higher cell densities at a higher growth rate than aged cells (PDL 77). Human diploid fibroblastic cell line, TIG-3, has a finite life span of about 80 population doubling levels (PDL), and is used for in vitro aging studies.
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